We are continuing our efforts to enhance the accessibility of the website as much as possible, out of our moral obligation to enable the use of the website for the population as a whole, including people with disabilities. *FyPYj`%;{{| X[-cr#WsGcOj2|94b R)U.\+VTUa;'19I&Q/hx^4mwhvM4'2#^>xkD[bur@,WLEnT4aUjuto7209g9C.8~nq|0\/i2746YSufy8!>;lLN&I6?Nf^"4|9JGBv.gBs Victoria Power Station, B. Wildtype primary B cells were treated with vehicle control (), 5 g/ml antikappa antibody or 1 M LatA for the indicated time. IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, H. Bagheri, H. Friedman, H. Shao, Y. Chong, C.-A. Available Conjugates The cells were first labeled with mouse anti-human CD3 antibody and then stained with goat anti-mouse IgG labeled with Compound No. ULTRA Series filter sets provide better Methods and devices for cytometric analysis are provided. Flow Cytometry Panel Builder This flexibility in laser wavelengths allows you to optimize assay design using the latest fluorescent dyes and substrates, Can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of todays assay requirements, The fluidics design ensures that the laser is precisely focused on the sample stream and maximum amount of emitted light can be collected for added sensitivity in multicolor applications, Fixed alignment also minimizes startup time, improves experiment-to-experiment reproducibility and enables automated daily quality control, The optional BD FACSFlow Supply System Fluidics Cart increases capacity and ease of use while maintaining a stable fluidics pressure. B. Hellenkamp, S. Schmid, O. Doroshenko, O. Opanasyuk, R. Khnemuth, S. Rezaei Adariani, B. Ambrose, M. Aznauryan, A. Barth, V. Birkedal, M. Bowen, H. Chen, T. Cordes, T. Eilert, C. Fijen, C. Gebhardt, M. Gtz, G. Gouridis, E. Gratton, T. Ha, P. Hao, C. Hanke, A. Hartmann, J. Hendrix, L. Hildebrandt, V. Hirschfeld, J. Hohlbein, B. Hua, C. Hbner, E. Kallis, A. Kapanidis, J.-Y. . A. Borgia, M. Borgia, K. Bugge, V. Kissling, P. Heidarsson, C. Fernandes, A. Sottini, A. Soranno, K. Buholzer, D. Nettels, B. Kragelund, R. Best, B. Schuler. 0000038853 00000 n T-cell infiltrates are not only found within the parenchyma and the meninges, but also in the cerebrospinal fluid (CSF) that bathes the entire CNS tissue . Y. Cheng, T. Stakenborg et al., Fluorescence Near Gold Nanoparticles for DNA Sensing, Anal. Luke Summer House Ex Girlfriend, Request a quote White, S.V. Flow cytometry combines the flexibility and sensitivity of fluorescence technology with high speed and data integration capabilities. Telefon: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: orders@antikoerper-online.de ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates The dye is moderately hydrophilic. Suitable for flow cytometry in the AmCyan channel Highly water soluble and pH-insensitive. X. Chen, T. Liu, X. Qin, Q. Nguyen, S. Lee, C. Lee, Y. Ren, J. Chu, G. Zhu, T.-Y. Converse Library Sample, FluoroFinder All Rights Reserved. 0000031395 00000 n Shipping costs, Terms and Conditions JMRK%\Of&-:\m;DQ8_T,9EXeM'gJ Qi^Fd-j*E65l) }%$%o^? Bode Plot Solved Examples In Control System Pdf, Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes The link to this site neither makes nor implies any representation or warranty for any products or services offered on a third-party site and is intended only to enable convenient access to the third-party site and for no other purpose. Clicking on the menu opens accessibility buttons. BioSyst. Techniques for flow cytometer alignment - PubMed Acids Res. Mater. PDF Product Information: ATTO 550 Special Order Research Product (SORP) Program for Custom Solutions New developments in illumination sources, digital signal processing and microsphere chem. All this results in the ultimate flow cytometry solution for deep immunoprofiling, from 24 . This website is run by the accessibility program of the "Accessible with a Click" company and is run via a designated accessibility server. Affinity purified on immobilized antigen. The fluorescence is excited most efficiently in the 540 565 nm range. xref This filter set is also ideal for obtaining high signal-to-noise ratios for TAMRA probes used in real-time qPCR. M. Pazos, K. Peters, M. Casanova, P. Palacios, M. VanNieuwenhze, E. Breukink, M. Vicente, W. Vollmer, Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division, Nature Communications 9, 5090 (2018). If you are having trouble resolving a population in a channel, especially one close to a laser line, it may be worth investigating a laser light leakage issue into that channel. FluoroFinder Chem. ATTO 550: 554 576 Details ATTO 565: 563 592 Details . 550 600 e (cm-'M-') 1960000 240000 116000 239000 Quantum Yield 0.68 0.90 Brightness Brightness intensity 3420000 1120000 163200 104400 78870 655 575 660 603 573 668 . 0000190655 00000 n Warrantee of use applies to the website owners and/or their representative, including the content displayed in the website, as subject to the conditions of use. %%EOF The PLT-F channel can be selected for testing on any sample or only used as a reflex test if the RBC or platelet size histograms are abnormal or if the platelet count is below a preset limit (determined by the user). J. Wardyn, A. Chan, A. Jeyasekharan, A Robust Protocol for CRISPR-Cas9 Gene Editing in Human Suspension Cell Lines, Current Protocols 1, e286 (2021). This dye is highly suitable for direct flow cytometry (FACS) using the He:Ne laser. Maximum absorption 601 nm; Maximum fluorescence 627 nm. Em. NOVEL POLYPEPTIDES AND USES THEREOF - patents.justia.com 0000214115 00000 n Intracellular and Plasma Membrane Cholesterol Labeling and - PubMed For more country-specific shipping and contact information see Ordering & Shipping. 0000224175 00000 n Y. Chen, S. Aslanoglou, T. Murayama, G. Gervinskas, L. Fitzgerald, S. Sriram, J. Tian, A. Johnston, Y. Morikawa, K. Suu, R. Elnathan, N. Voelcker, Silicon-Nanotube-Mediated Intracellular Delivery Enables Ex Vivo Gene Editing, Advanced Materials 32, 2000036 (2020). 83, 1307 (2011). Luke Summer House Ex Girlfriend, The XN-550 features an automated sampler and so improves workflow productivity with its Rerun & Reflex functionality and continuous loading feature. This page has been recently translated and is available in French now. Yang, E. Cooper, B. Chen, K. Siminovitch, A. Peterson. [I%k Chen, W.-Y. M. Urban, S. Both, C. Zhou, A. Kuzyk, K. Lindfors, T. Weiss, N. Liu, Gold nanocrystal-mediated sliding of doublet DNA origami filaments, Nature Communications 9, 1454 (2018). 0000020039 00000 n A. J. Nikolic, L. Belot, H. Raux, P. Legrand, Y. Gaudin, A. Albertini, Structural basis for the recognition of LDL-receptor family members by VSV glycoprotein, Nature Communications 9, 1029 (2018). - ATTO 550 absorption (.txt), Absorption and Emission Spectrum (graphic) Flow cytometry was used to determine T cell phenotype and ion channel expression. It is used to identify and gate cells in the context of data spread due to the multiple fluorochromes in a given panel. We aim to ensure that digital services are accessible to people with disabilities, and therefore we have invested large resources in order to simplify the use of the website for people with disabilities as much as possible, out of the belief that every person deserves the right to live with equality, dignity, convenience, and independence. It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta. ATTO-550 (554/576) and ATTO-620 channel. Surawski, B.J. Changing color contrast based on light backgrounds D. Daems, W. Pfeifer, I. Rutten, B. Sacc, D. Spasic, J. Lammertyn. 0000191226 00000 n As expected, the addition of the amphiphile triggered the appearance of fluorescent pixels in the red channel of the confocal fluorescent microscopy images (Fig. Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers. M. Chung, D. Kim, A.E. BD flow cytometers are Class I (1) laser products. E. Ronzitti, B. Harke, A. Diaspro, Frequency dependent detection in a STED microscope using modulated excitation light, Optics Express 21, 201 (2013). It includes an automated sheath and waste fluid control system that reduces daily maintenance by incorporating two 20-L containers (Cubitainers), Fluidic sensors maintain constant pressure, while a fluidics monitoring system warns when sheath fluid is low or empty or when the waste container is full. Chem. 550/40 VL2 512/25 VL2(V6) 525/50 eFluor 506 Pacific Green LIVE/DEAD Fixable Aqua . " /> 0 4, 1000134 (2013). Avenue Jules Bordet 160 16, 1140 Evere - Belgium Phone: +32 2 31 50 800 Fax: +32 2 31 50 801 E-mail: info@kyvobio.be Antibodies allow selective detection of specific proteins. Miller, R. Vogel, P.P.T. 40, 5368 (2012). your query. Product availability and prices are subject to change without notice. 0000196962 00000 n Kim, G. Krainer, D. Lamb, N. Lee, E. Lemke, B. Levesque, M. Levitus, J. McCann, N. Naredi-Rainer, D. Nettels, T. Ngo, R. Qiu, N. Robb, C. Rcker, H. Sanabria, M. Schlierf, T. Schrder, B. Schuler, H. Seidel, L. Streit, J. Thurn, P. Tinnefeld, S. Tyagi, N. Vandenberk, A. Vera, K. Weninger, B. Wnsch, I. Yanez-Orozco, J. Michaelis, C. Seidel, T. Craggs, T. Hugel, Precision and accuracy of single-molecule FRET measurementsa multi-laboratory benchmark study, Nature Methods 15, 669 (2018). A.-K. Schneider, T. Scharnweber, D. Cammann, B. Rapp, S. Giselbrecht, C. Niemeyer, Multiscale Microstructure for Investigation of CellCell Communication, Small Methods 4, 2000647 (2020). When setting up use the voltage setting to increase autofluorescence in the BV510 channel,. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Ffx Qactuar Monster Arena, A core lab workhorse providing power, performance and consistency. D. Bracha, M. Walls, M.-T. Wei, L. Zhu, M. Kurian, J. Avalos, J. Toettcher, C. Brangwynne, Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds, Cell 175, 1467-1480.e13 (2018). Fluorescent labbeled antibody detected in a Flow Cytometer. Antibody conjugation is a critical step in many molecular-biology research assays. Park, I. Jeon, B. 0000253490 00000 n 42 0 obj <>/Filter/FlateDecode/ID[<9473BD190E70408FBB7CCF0FFC9676FA>]/Index[9 57]/Info 8 0 R/Length 144/Prev 546667/Root 10 0 R/Size 66/Type/XRef/W[1 3 1]>>stream B. Dalzon, A. Torres, H. Diemer, S. Ravanel, V. Collin-Faure, K. Pernet-Gallay, P.-H. Jouneau, J. Bourguignon, S. Cianfrani, M. Carrire, C. Aude-Garcia, T. Rabilloud, How reversible are the effects of silver nanoparticles on macrophages?, Environmental Science: Nano 6, 3133 (2019). Merged image The exact immunogen sequence used to generate this antibody is proprietary information. 0000190838 00000 n 14, 4707 (2014). Low carryover is essential in research applications to ensure sample purity and data integrity. Irving et al., Reactive centre loop mutants of -1-antitrypsin reveal position-specific effects on intermediate formation along the polymerization pathway, Biosci. ATTO 550 is a cationic dye. P.P.T. Due to the spatial limitations of flow cytometry when imaging, spectral imaging is conducted by selecting a smaller region of interest (usually having the dimensions of a single cell) and restricting the number of wavelength bands that are gathered. Sung, M.-J. The Fluorescence Minus One Control, or FMO control is a type of control used to properly interpret flow cytometry data. 18,27 We also investigated the use of flow cytometry to quantify the amount of ht-GFP. Le Marois, K. Suhling, D. Richards, A. Zayats, Frster Resonance Energy Transfer inside Hyperbolic Metamaterials, ACS Photonics 5, 4594 (2018). J. Scholefield, R. Henriques, A. Savulescu, E. Fontan, A. Boucharlat, E. Laplantine, A. Smahi, A. Isral, F. Agou, M. Mhlanga, Super-resolution microscopy reveals a preformed NEMO lattice structure that is collapsed in incontinentia pigmenti, Nature Communications 7, 12629 (2016). Fluorescent ATTO Dyes | Alomone Labs Looks like you're visiting us from {{countryName}}. M. Singh, M. Watkinson, E. Scanlan, G. Miller, Illuminating glycoscience, RSC Chemical Biology 1, 352 (2020). 0000022708 00000 n Special Topics 199, 181 (2011). Overview of Flow Cytometry Reagents Mix-n-Stain Antibody Labeling Kits Apoptosis Assays Dead Cell Stains Proliferation & Viability Assays Cell Cycle Analysis Flow Cytometry Accessory Products View all in Flow Cytometry BACK Overview of CF Dyes & Other Bioconjugates Annexin V Conjugates Alpha Bungarotoxin Conjugates J. Nikolic, L. Belot, H. Raux, P. Legrand, Y. Gaudin, A. Albertini. 0000033916 00000 n D. Rutz, Q. Luo, L. Freiburger, T. Madl, V. Kaila, M. Sattler, J. Buchner. Luke Summer House Ex Girlfriend, S. Nasrin, A. Rashedul Kabir, A. Konagaya, T. Ishihara, K. Sada, A. Kakugo, Stabilization of microtubules by cevipabulin, Biochemical and Biophysical Research Communications 516, 760 (2019). ATTO and alternative dye - Bio-Syn Generation of ATTO-labeled -syn. S. Baliga, C. Murphy, L. Sharon, S. Shenoy, D. Biranthabail, H. Weltman, S. Miller, R. Ramasamy, J. Shah. Sitemap, ISO 9001:2015 Spectral flow cytometry, an emerging methodology that is not confined by the "one color, one detector" paradigm, shows promise in the facile detection of multiple fluorescent proteins. N. Hazan, T. Tomov, R. Tsukanov, M. Liber, Y. Berger, R. Masoud, K. Toth, J. Langowski, E. Nir, Nucleosome Core Particle Disassembly and Assembly Kinetics Studied Using Single-Molecule Fluorescence, Biophysical Journal 109, 1676 (2015). The BD LSRFortessa Cell Analyzer has been effective in the analysis of Treg populations and T cell subtypes. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva. The octagon- and trigon-shaped optical pathways of collection optics maximize signal detection and increase sensitivity and resolution allowing you to identify dim and rare cell populations Can be configured with up to 5 lasersblue, red, violet, UV and yellow-green. Tel: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: orders@anticorps-enligne.fr Search results for ATTO Antibody at Sigma-Aldrich. Belongs to the class of Rhodamine dyes. W. Ren, S. Wen, S. Tawfik, Q. Su, G. Lin, L. Ju, M. Ford, H. Ghodke, A. van Oijen, D. Jin. Adapting the website to color blind people Comm., 4783 (2005). C 114, 4345 (2010). Avenue Jules Bordet 160 16, 1140 Evere - Belgium Phone: +32 2 31 50 800 Fax: +32 2 31 50 801 E-mail: info@kyvobio.be ULTRA Series Cy3 fluorescence filter set designed to provide bright, high-contrast images of Cy3-stained samples. 0000276406 00000 n This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. J.N. Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. -ATTO 550 product information, - NHS-ester selection guide for flow cytometry Excitation laser Common emission filters (nm) Attune NxT channel (nm)* Recommended dyes . " />, Call Us: Miami (305) 649-5344 / CALL FREE: 800-910-8378 Hialeah Gardens (305) 822-0666 | info@cdltmds.com | My Account. 550/30 TagYFP: 508: 524: 488, 514, 532: . J. de Torres, M. Mivelle, S. Moparthi, H. Rigneault, N. van Hulst, M. Garcia-Parajo, E. Margeat, J. Wenger, Plasmonic Nanoantennas Enable Forbidden Forster Dipole-Dipole Energy Transfer and Enhance the FRET Efficiency, Nano letters 16, 6222 (2016). Two levels of system alignment are . Protect from light. we$AJ_-YD5S? This experiment was performed under reducing conditions using the 12-230 kDa separation system. Syeda Rubaiya Nasrin, Tsukasa Ishihara, Arif Md. D. Daems, W. Pfeifer, I. Rutten, B. Sacc, D. Spasic, J. Lammertyn, Three-Dimensional DNA Origami as Programmable Anchoring Points for Bioreceptors in Fiber Optic Surface Plasmon Resonance Biosensing, ACS Applied Materials & Interfaces 10, 23539 (2018). Flow cytometry is a technique used to analyze cells for a variety of purposes, including cell counting, phenotyping, cell cycle assessment, and viability. Victoria Power Station, PDF Fluorochrome/Laser Reference Poster - University of Iowa G. Lin, M. Lewandowska, Plasmon-enhanced fluorescence provided by silver nanoprisms for sensitive detection of sulfide, Sensors and Actuators B: Chemical 292, 241 (2019). It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta. Easy visualization of some of the most popular lasers and filters across the fluorescence spectra. S. Mukherjee, J.-M. Knop, R. Oliva, S. Mbitz, R. Winter, Untangling the interaction of -synuclein with DNA i-motifs and hairpins by volume-sensitive single-molecule FRET spectroscopy, RSC Chemical Biology 2, 1196 (2021). "> Spectra Viewer Select machine + Add Fluorophore Fluorophores Ex. 2023 Alomone Labs. Ffx Qactuar Monster Arena, 2 Images : +351 30 8808 050 Fax : +351 30 8808 052 info@quimigen.pt www.quimigen.pt Expression of TRPV4 in rat DRG primary culture - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture.A D. Staining usingAnti-TRPV4Antibody (#ACC-034) (1:500) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.B E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.C. Spectral Viewer - Beckman L. Cruz, T. van Dijk, O. Vepris, T. Li, T. Schomann, F. Baldazzi, R. Kurita, Y. Nakamura, F. Grosveld, S. Philipsen, C. Eich, PLGA-Nanoparticles for Intracellular Delivery of the CRISPR-Complex to Elevate Fetal Globin Expression in Erythroid Cells, Biomaterials 268, 120580 (2021). In a-PBTs, in addition to K + channel activity and Ca 2+ fluxes, chemotaxis was measured. Long, K. Ubych, E. Jagu, R. Neely, FRET-Based Method for Direct, Real-Time Measurement of DNA Methyltransferase Activity, Bioconjugate Chemistry 32, 192 (2021). Cell cycle progression was investigated by flow cytometry of DNA content. K. Gpfrich, M. Urban, C. Frey, I. Platzman, J. Spatz, N. Liu, Dynamic Actuation of DNA-Assembled Plasmonic Nanostructures in Microfluidic Cell-Sized Compartments, Nano letters 20, 1571 (2020). 0000196881 00000 n These molecules are very useful in flow cytometry, because of their brightness, they excite well with the typical 488 nm laser line, and can serve as efficient FRET donors to near-infrared dyes. To add one or more excitation sources, click "Excitation Source" in the "Add" submenu on the left part of the screen. This label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. 0000190721 00000 n 1 Quantum yield added where available; the quantum yield and fluorescent lifetimes can be highly dependent on the local environment. These molecules are very useful in flow cytometry, because of their brightness, they excite well with the typical 488 nm laser line, and can serve as efficient FRET donors to near-infrared dyes. Y. Li, J. Bolinger, Y. Yu, Z. PDF Introduction to flow cytometry - Abcam D. Bracha, M. Walls, M.-T. Wei, L. Zhu, M. Kurian, J. Avalos, J. Toettcher, C. Brangwynne. Products Learn Support Quality About Us Contact Us Custom Solutions Custom Reagents Custom Services Custom Requests Form Login/Register (0) Menu Login/Register (0) The HTS Option 0000021294 00000 n Northland College Women's Hockey Roster, J. Funke, H. Dietz, Placing molecules with Bohr radius resolution using DNA origami, Nature Nanotechnology 11, 47 (2016). Product Sheets 0000190334 00000 n LSRFortessa | High-Parameter Flow Cytometer - BD Biosciences U. Chio, S. Chung, S. Weiss, S.-O. 510/550 (32012A) 615/740 (32015A) 665/685 (32013A) Designed for use in spectral flow cytometry, to fill in gaps between common fluorophores . I. Hoffecker, S. Chen, A. Gdin, A. Bosco, A. Teixeira, B. Hgberg, SolutionControlled Conformational Switching of an Anchored Wireframe DNA Nanostructure, Small 15 (2019). Changing color contrast based on light backgrounds Up to 14 parameters from 4 lasers 0000186734 00000 n Flow Cytometry Analysis. %PDF-1.7 % This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. introduction Omega Optical. S. Lee, J.-H. Bong, J. Jung, J. M. Chai, S. Razavi Bazaz, R. Daiyan, A. Razmjou, M. Ebrahimi Warkiani, R. Amal, V. Chen, Biocatalytic micromixer coated with enzyme-MOF thin film for CO2 conversion to formic acid, Chemical Engineering Journal 426, 130856 (2021). HTS provides rapid, fully automated sample acquisition from 96- and 384-well microtiter plates. ATTO 550 Orange 554 576 791 Cy3.5 Orange Red 581 596 1,286 microscopy, flow cytometry and immunohistochemistry. S. Hou, L. Sun et al., Fluorescence correlation spectroscopy analysis for accurate determination of proportion of doubly labeled DNA in fluorescent DNA pool for quantitative biochemical assays, Biosens. Luke Summer House Ex Girlfriend, 0000276147 00000 n View theBD LSRFortessa System brochure. Adapting the website to color blind people Commun. Any advice on Auto fluorescence- Flow cytometry-Free Channel J. Spitzberg, X. van Kooten, M. Bercovici, A. Meller, Microfluidic device for coupling isotachophoretic sample focusing with nanopore single-molecule sensing, Nanoscale 12, 17805 (2020). Tomov et al., Detailed Study of DNA Hairpin Dynamics Using Single-Molecule Fluorescence Assisted by DNA Origami, J. Phys. S. Qin, S. Isbaner, I. Gregor, J. Enderlein, Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy, Nature Protocols 16, 164 (2021). M. Barbiero, S. Castelletto, Q. Zhang, Y. Chen, M. Charnley, S. Russell, M. Gu, Nanoscale magnetic imaging enabled by nitrogen vacancy centres in nanodiamonds labelled by iron-oxide nanoparticles, Nanoscale 12, 8847 (2020). Please fill in the following information and we will get in touch with you regarding your query. Adding a dump channel to your panel design is easy! ATTO-TEC GmbH Martinshardt 7 D-57074 Siegen Germany Phone: +49 271 23853 - 0 FAX: +49 271 23853 - 11 E-mail: info@atto-tec.com http: www.atto-tec.com Revised: 2022-12-13 ATTO 550 is a novel fluorescent label related to the well-known dye Rhodamine 6G. P. Comba, A. Eisenschmidt, L. Gahan, D.P. J. Strmqvist, L. Nardo et al., Binding of Biotin to Streptavidin: A combined fluorescence correlation spectroscopy and time-resolved fluorescence study, Eur. Figure 8. Fluorescent Proteins for Flow Cytometry - PMC - National Center for (PDF) Warfarin overactivity | volkan inal - Academia.edu How it works The membranes of the platelets are perforated by the lysing reagent but they remain largely intact during this process. . 0000190962 00000 n Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. T` GDbqb~Jh!7}IXc-tOa^ Recombinant -syn protein was purchased in a lyophilized form from Alexotech in a lyophilized form. ATTO 550 is a cationic dye. 0000307867 00000 n ATTO 550 is a fluorescent label related to the well-known dyes Rhodamine-6G and Rhodamine B, the commercial alternative to NEDTM. Maximum signal, minimum crosstalkan innovative and proven platform for multicolor analysis. 0 0000223755 00000 n 0000214486 00000 n 0000005696 00000 n XN-550 incorporates the proven Sysmex technologies of fluorescence flow cytometry, hydrodynamic focussing . Lo, F. Emran, I. Kays, X.-J. Intracellular flow cytometry BUV395 is designed for instruments equipped with a 355 nm UV laser and a 379/28 filter. 436 0 obj <> endobj B. Hellenkamp, S. Schmid, O. Doroshenko, O. Opanasyuk, R. Khnemuth, S. Rezaei Adariani, B. Ambrose, M. Aznauryan, A. Barth, V. Birkedal, M. Bowen, H. Chen, T. Cordes, T. Eilert, C. Fijen, C. Gebhardt, M. Gtz, G. Gouridis, E. Gratton, T. Ha, P. Hao, C. Hanke, A. Hartmann, J. Hendrix, L. Hildebrandt, V. Hirschfeld, J. Hohlbein, B. Hua, C. Hbner, E. Kallis, A. Kapanidis, J.-Y. 0000030893 00000 n Galifornia Wholesale Phone Number, 0000096953 00000 n 0000031755 00000 n Contact our Technical and Applications Supportpersonnel for maintaining optimal instrument performance and with any other instrument-related support. 0000213629 00000 n Flow cytometry studies are used to identify and quantify immune cells and characterize hematological malignancies.1 They can measure: cell size. 0000003399 00000 n 0000003664 00000 n DAPI | Cell Signaling Technology Note: If a filter is added to the graph, a new column will appear in the information table at the bottom of the page, labeled "Spillover" with the filter shown in parentheses.