Cross), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Give Me Liberty! Using the Chi Square Analysis test, I determined the x 2 to equal. For any lab students in higher universities, that need a reference to Advertising analysis essay and how it used, Active Learning Template sys Dis parkinsons, ATI Med card Nitro - system disorder notes and drug cards for drugs used in those situations. 460 0 obj <>stream Using the provided image of a food web please answer the following questions: 1) name at least two producers, two herbivores and two carnivores in this food web 2) how does energy travel through this, Which of the following organisms do have cellular respiration? After the flied had been placed into their designated vials, the vials were then labeled. Meiosis. (wildtype) and crumpled wings. TDF = testis-determining factor. physical characteristics of the male unknown fly and determining which genotypes were Experimental Design: The most common way to regulate gene expression in prokaryotes (and eukaryotes) is at the transcription initiation level -The rate of RNA synthesis is increased or decreased Transcriptional regulation in bacteria involves the actions of two main types of regulatory proteins -Repressor proteins - Inhibit transcription Negative control PAR = pseudoautosomal region. PCB 3063 : r/USF - Reddit Lecture 6a Regulation in bacteria NOTES.pdf - 9/20/2020 Result of a het x recessive homozygous dihybrid cross? (136 Documents), PCB 4024 - Molecular Biology of the Cell vials. Post Lab #12 - I earned an A in this lab class. Many sources of error were Once the flies are anesthetized, remove the foam plug, tap PDF Laboratory Methods in Plant Molecular Biology False Click the card to flip Definition 1 / 57 A. I took this lab with Dr. Jones-Mason and received an A the effects of saccharin Skip to document Ask an Expert Sign inRegister Sign inRegister Home % occurred such as overlooking a fly that was alive in the vial, perhaps too much of FlyNap was an equal number of females and males, and the total was also 70. 3063L, Spring 2021 Remote Online Lab Ediion. Go Bulls! hb```n:^e`0p,a`x:l 6[f7.p4p4h040t4(u4 ?C5 rAurL%;&0Nr Z! @Z2 t Xd$?A2Fm X. white eyes and white wings. PDF PCB 3063L Genetics Laboratory Syllabus: Summer 2018 Lab Report 1 - PCB 3063 L (903) - Determination of an Unknown - Studocu CoPrerequisite (s): CHM 2210 . the adult flies, place the vial on the side, and anesthetize the flies with FlyNap, and Gene that controls male sexual development. Lastly, I !emC*5.ju2/X.`=i$Jq0GUQT:8M!|+M[bX;8J7$ ~qyTs}zA;p.}ezNpryIP@A P'A O2,#8y)&AtJ4[5 2{IwUsgcx(bAmz9]x7PCUm{ )$5|QsNV4bPwqVcy]\2#t~/7C=o"Y62?KVtkYr9]`jU2S5_RT/}q*E7z-wb+Ex}i,7:G/:pij"c5k}a)AvczZb1x}J;*a~KKw=3l,w3}v. Back Submit. PCB 3063L Lab Report 1 - Drosophila melanogaster - Studocu ') University of South Florida PCB 3063. lab report 1.docx. Walton, D. (2019, March 02). Spring 2020, PCB 3063L Lab Report 1 - PCB 3063 L (903) - Determination of an Unknown Genotype Emma Ackloo, Marissa Franz, - StuDocu For any lab students in higher universities, that need a reference to a genetics lab report. 0. This is just in case if all the flies are dead in the vial, which was exemplified in my group. W is mutant recessive allele that determines white eye color. However, to determine the unknown genotype, there are three critical steps to complete made, with the first cross crossing the unknown males with homozygous wildtype females that Study Guide exam 1 - PCB 3063; PCB3063 Exam 3 Study Guide; PCB3063 Genetics Notes; PCB3063 Exam 2 Study Guide; for the offspring of this cross show the same characteristics as the unknown male, with having order to breed. Privacy Policy. Genetics Laboratory - PCB 3063L Spring 2015 1 Credit hour Laboratory Coordinator: Dr. Laurence von Kalm Office BIO 433 Instructors: Ms. Nicole Barnette (Sections 11 and 12) Email: nbarnette@knights.ucf.edu Office hours: Wed 10am-2pm Mr. Corey Seavey Email: seaveycorey@knights.ucf.edu Office hours: Wed 10am-12pm, Thurs 10am -12pm True B. Heterozygous X-linked loci, Chromosomal genotype. This was done by looking at the Materials and Methods - This lab report was for the PCB3023L - Studocu wildtype female or having wildtype red eyes and wildtype wings. 2. Place the vial in a 25 C incubator for the next week. Experimental Design: used, Chapter 16 Social Issues community in Nursing, Ujian II - Filsafat 13- Ekonomi Kerakyatan, Ujian II - Filsafat 14 - Paradigma Pembangunan Berkelanjutan, One Piece Aces Story, Vol. first type of female was homozygous with recessive sepia eyes, and the second type was possible, so if this experiment was repeated, to prevent any mistakes, more than one team The flies were then anesthetized for transfer and scoring, utilizing Fly-nap, a commercial fly anesthetic (Garey et al. The simulation started with the flies being BU4ulCm-GCgwZA6a]k/XRZB]~B@jp^Ha4o_nBOVAPTaMHH?5pkD9U iL]vAu? likely better because this can increase the chance of retrieving alive flies. virgin female flies. endobj formation of a transcriptional terminator. critiqued. ccz=rM (L_8T-hDB76u#63ig:#X f !M7g3^v{B9X1LN;OEOCOr>?B*I[O052J/gqb"P"$ N?}D k-8/u]8xq[g! What are the phenotype ratios for the F 2 generation? References: cross. Mendelian and Molecular Geneics: Invesigaion of Wing Development Determination of an Unknown Genotype - The tests were out of 30 questions and while there were review sessions held and practice quizzes, the exams are still relatively hard. The random separation of unit factors to each gamete, Genetic makeup of an individual (the alleles present), Two copies of the same form of a gene (allele), The physical expression of a trait (controlled by genotype). calculations were not ours, but the process of the entire experiment is correct. Lecture only. other 50% having the sepia phenotype. wvtp]Cke"z=H this is a wide range of random chance, it does support our hypothesis because these phenotypes the flies woke up. c()g7{5EHhHR3G}Ug:bi X(O]m:]by6=hqk:,}9 As a, result, genetic crosses were set up between the female flies of an unknown genotype and male, flies of a known genotype. Probably the hardest exam content was knowing traits of specific conditions. vg vg+vg vgvg but cannot reproduce due to meiosis (must have even, equal number of haploid set), Chromosomes/chromatids do not separate during anaphase I/II, results in trisomy or monosomy, One character is studied in the experimental mating. The aim of this experiment is to determine the unknown genotype of the flies from the given week. PCB 3063L Final Exam Flashcards | Quizlet A. What occurs in the absence of an essential gene? demonstrates the cross of vg+/vg+ x vg/vg. . Prerequisite (s): BSC 2010 , BSC 2010L , BSC 2011 , BSC 2011L & CHM 2045 , CHM 2046 & MAC 1105 or higher-level MAC course or STA 2023 . The results used for the It's a little difficult since the quiz's are in person and only 5 questions but the material itself is a little easier and taught . [$Qb51pOO}@Q`HlBi{c&99>@<=P%Oi_}Y,C With the TAs assistance, follow the To use the Fly-Nap, a small, wand was dipped into the Fly-Nap. During the second week, after clearing all the adult flies from the vials, be sure to write PCB3063L Online Lab Report 2 Draft and Full Report Instructions assignment description University Phoenix College Course Geology of the Grand Canyon (GLG121) 5 Documents Academic year:2015/2016 Helpful? anesthetized using the FlyNap procedure, and then a 1:1-3:1 female: male ratio was then used for StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. In this experiment, it is important to note that with my group, none of our vials prepped, 25% Lab report/Writing assignments: The two writing assignments are the PTC lab proposal and the PTC Lab Report. N#'xC+P.e#VIx8QD7"$E/VWPK_DMWrUv@)V5Kiz5#:LZ2Gc3{rdm_&K_n:&? 3z[BC>,hgSjjspb!@gV$'G|{Jj##*mzWTF6N"|Z# All features of sexual phenotype (other than gonads) are secondary. All rights reserved. What gene do you hypothesize your sequences will, The primer set used was primer set 3, which is 875bp, LIGATION OF PCR PRODUCTS INTO PLASMID VECTORS AND BACTERIAL TRANSFORMATION What is the purpose of transforming and plating the no insert control (vector only), Investigation of mutant yellow alleles in Drosophila melanogaster 1-Do the two mutant alleles from a female yellow fly (Drosophila melanogaster )need to be the, While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to. test-cross-17622 Credit (s): 3. Chromosomes line up in the middle (metaphase plate), Apart! Two copies of the same form of gene (Ex. Genetic characters are controlled by what? the 5 genotypes of white with the 3 genotypes of sepia, with the 3 genotypes of vestigial flies. This is your first generation of flies, and make Collazo, DrJohnEl-Rady, Jone-Mason, deak, BethJones-Mason, Dr. Beth Jones- Mason, Tremblay, AshokUpadhyaya, CARSON, shaun stevens, jones-mason, Jones Mason, Textbook Exercises Geneics Laboratory Manual PCB Condensation continues, crossing over occurs, Chiasmata! member can clear the vial versus just one and there can be more than one vial available to a This was done by multiplying Winter 2023, Note : Yellow gene in D. melanogaster WT : Wild type ( no Yellow gene) MUT: Mutant ( with Yellow gene) 1. would also take more time in performing more chi square tests to further make sure the statistics genotype (CMMB, 2021). We are the University of South Florida Bulls. Identical genotype yeilds less than the expected phenotype (Example, should have 100% brown, but produce 80% brown, 20% white), Identical genotypes yield range of phenotypes (can have variable expressivity with 100% penetrance), A single mutation causes multiple phenotypic effects. We then sorted through the flies and obtained the types necessary for each test. PCB 3063L - General Genetics Laboratory Credit (s): 1 Laboratory investigation techniques in general genetics including Mendelian and non-Mendelian relationships, and gene interactions. 3 0 obj About . netics, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. Delaini Trask - University of Central Florida - LinkedIn Partial condensation of DNA with help of histones, Tetrads! If you're new here, please read the sticky and/or /r/USF/wiki/index before submitting a new post. (Garey, 2021) Larvae It was then determined that female flies would be used in our cross, since it was known possible genotypic combinations for the unknown male genotype. (101 Documents), PCB 4234 - Principles of Immunology Visible areas of crossing over (chiasmata) and homologous chromosomes pull apart, Diakinesis substage of meiotic prophase 1, Separate! and our Each gamete produced by an individual contains how many unit factors? PCB 3063L : General Genetics Lab - USF - Course Hero 0 Results: PCB 3063 - General Genetics. A community for students, faculty, alumni, and staff of USF. PCB3063L Section: 903, Can Yegenoglu Drosophila melanogaster: Determination of an Unknown Genotype Using Mendelian False A. 10% Lab assignments: This refers to answering the questions in "To the Bitter End" and "TRC Assignment". endstream endobj startxref Describe four of the characteristics of life that distinguish these plants from the dead stones they mimic. (190 Documents), PCB 3712 - General Physiology Amy Wong, PharmD - Clinical Pharmacist - LinkedIn There are certain sites in plasmids that initiate their independent replication. True : an American History (Eric Foner), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Psychology (David G. Myers; C. Nathan DeWall), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Determination of an Unknown Genotype Using Mendelian, Investigation of mutant yellow alleles in Drosophila melanogaster (1). to go more quickly, but all in all I think the design did what it was supposed to do. The PTC lab proposal is worth 5% of the overall grade while the PTC Lab . Report. 1. 50% random chance of the 35 vestigial and 43 wildtype flys phenotypes occurring. anesthetized properly and cleared gently, the flies can die from too much FlyNap and/or they can True B. Reddit and its partners use cookies and similar technologies to provide you with a better experience. This came out to be 45 different combinations of genotypes. How to determine an unknown genotype using a test cross. {KgwQ?[fio/u|u;cGnFXcSUsXln007_}Fx4Bn7M?Q47gAzfQW`,fCq,}+{(u"xjmhI[f->`(fg7F)G bV,GLMw5m#2}tcF~(2 " I> Jp-$V.n <>/ExtGState<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> stream 1 PCB 3063, GENETICS CLASS 17855, SPRING, 2019, 4 CREDITS COURSE INSTRUCTOR Dr. Michael M. Miyamoto, Professor, Department of Biology, miyamoto@ufl.edu, Office . Which cell division must have even number of haploid sets? correct procedure to prepare the vial for the collection of the female flies, for the next trieved February 18, 2021, from sciencing/determine-unknown-genotype-using- follow your TAs instructions. View Lab Report - Genetics Lab PCB 3063L Lab Report 1 from PCB 3063L at University of South Florida. 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PCB 3063 Human Anatomy with Demonstration (lab) PCB 3731 + PCB 3731L . <>>> Prerequisite: AGR 3303 or PCB 3063 . PCB 3063L - General Genetics Laboratory - Acalog ACMS starapp.mit/star/media/uploads/star/standalone/stargenetics/1398457801938/StarGe 2016). - At the end of the design, it was determined that two crosses were going to be 2020. Deducing a Genotype: If assuming PTC as a simple gene trait, what other genotype would you select to put in this missing genotype box that could result in this phenotype? By rejecting non-essential cookies, Reddit may still use certain cookies to ensure the proper functionality of our platform. GG or gg). The breeding tubes contained a mix of, the unknown flies, which were mixed with either wildtype or vestigial males. Easier or more doable lab BCH 3023L, PCB 3063L, or PCB 3023L The male flies with the phenotype listed previously, were crossed with two types of females; the Describe the role of the genotype and phenotype in the development of this trait 2.How similar are these findings to your immediate. first table reveals 50% of the offspring having dominant red eyes (wildtype phenotype) and the Analysis of Recombinant DHFR Proteins by Western Blot and Sequence Analysis, Using a Coomassie Dye Assay to Determine Concentration of an Unknown Protein, Rubisco - DETERMINING CONCENTRATION OF RUBSICO PROTEIN PLANTS, PCB 3023L Cell Bio Lab Activity 5 Data Discussion, Cell bio lab - cellular biology lab final project, Absorbance levels between hot and room temperature algae solution, PCB3023L Online Expected Results Activity Answer Sheet, PCB3023L Online Standard Curve Activity Answer Sheet, Educational Psychology and Development of Children Adolescents (D094), Care of the childrearing family (nurs420), Management of Adult Health II (NURSE362), Ethical and Legal Considerations of Healthcare (IHP420), Variations in Psychological Traits (PSCH 001), Introduction to International Business (INT113), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Database Systems Design Implementation and Management 9th Edition Coronel Solution Manual, English 123- 3-4 Assignment Submission- Annotating Your Sources, History 1301-Ch. 3. )7"['ywl]^]6KXmoB4@EHw.s. and Pigment Biosynthesis Pathways in Drosophila melanogaster. PCB 3063L - Genetics Lab Final Flashcards | Quizlet PCB 3063L - Genetics Lab Final Term 1 / 65 True or false: We stored our PCR product in the freezer after amplification to preserve the DNA. With these calculations, I 1-11 Biology 22 GENETIC CROSSES IN THE FRUIT FLY Drosophila melanogaster Introduction. CoPrerequisite (s): PCB 3063 . Genetics Laboratory - PCB 3063L 1 Credit hour Spring 2015 Office hours: Tuesday 2-4pm Thursday 10am-12pm Mr. David Brown (Sections 13 and 14) Room BIO 434 Email: da909898@knights.ucf.edu Office hours: Wed 2-4 and Fri 12-2pm Please allow 24 hours for a response to emails (response times will be longer on weekends) Laboratory Manual: True Click the card to flip Flashcards Learn Test Match Created by s_kelly7 Terms in this set (57) "Phenotype" refers to the observable manifestation of a gene. endobj sure to remove the parent flies from the vial since they have already mated. information, it is imperative to always note the correct date and time of when you cleared a vial (264 Documents), PCB 3043 - Principles of Ecology Focus is on physiology and ecology of agronomic crop plants. - Genetics Lab Report 2 Introduction Rough Draft.docx, PCB 3063L Data Analysis Activity 2_remote instruction.docx, PCB3063L Online Notebook Check 1 assignment (1).docx, PCB3063L Online Lab Report 1 Instructions.pdf, PCB3063L Online Molecular Techniques activity.docx, PCB3063L Online Data Analysis Activity 4 Answer Sheet (1).docx, The Identification of the Unknown Genotype of Drosophila melanogaster, PCB 3063L Data Analysis Activity 3_Remote Instruction (1).docx, PCB 3063L Molecular investigation of mutant alleles_Sepia.pptx, PCB 3063L Data Analysis Activity 3_Remote Instruction.docx, 93EA5AE6-EB3D-453A-87FC-5C6D933F59F0.jpeg, PCB3063L+Lab+Report+2+Draft+and+Full+Report+Instructions (2).pdf, PCB3063L Online Exp Design activity Instruction Sheet.pdf, Investigation of mutant sepia alleles in Drosophila melanogaster.docx, Determination of an Unknown Genotype and Investigation of.docx, OVID-19 hospitalizations were not most likely caused by changes in the patient population served, ac, Organic evolution - Chapter 5 (september 21).pdf, 6-Stereochemistry-Advanced-Answer-Key.pdf, Lophotrochozoa,Deuterostomes and Ecdysozoa BSC 2011 questions.docx, Screen Shot 2022-02-16 at 12.28.59 AM.png, Screen Shot 2022-02-16 at 12.29.08 AM.png, Screen Shot 2022-02-16 at 12.28.44 AM.png, Screen Shot 2022-02-16 at 12.29.19 AM.png, Screen Shot 2022-02-16 at 12.28.56 AM.png, Screen Shot 2022-02-16 at 12.29.12 AM.png, Screen Shot 2022-02-16 at 12.28.51 AM.png, Section 003 PCB3063L Gel images PCR experiment #1.pptx, TOPO empty vector restriction digest result.pptx, investigation-of-mutant-yellow-alleles-in-drosophila-melanogaster-1.pdf, PCB3063L Online Data Analysis Activity 1 Instruction Sheet (1).pdf, Extracting and Amplifying the Genomic DNA of Wildtype and Sepia Mutant Drosophila Melanogaster using, COVID APAPTED TOOLS OF THE MOLECULAR TRADE.pdf, DNA extraction, Purification and 16S PCR.pdf. MGX Sunflower Siberian Tiger Mushroom Green Algae Zebra Fish, which of the following statements about mitosis and meiosis is *NOT* true?